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Human Embryonic Stem Cell Line ESI-049 - (46,XY)

CAT #: ES-702

BioTime provides research grade versions of five independent embryonic stem cell lines that represent the best characterized and documented hESC lines available today. The ESI collection (ESI-017,035,049,051 and 053) was derived using procedures and documentation that are in compliance with cGTPs and cGMPs 1, and represents the most comprehensive collection of clinical grade lines available. Unlike other hESC lines, our ESI hESC lines were not developed using either live animal cells (such as mouse embryonic fibroblast feeder cells) or unqualified animal-based products for culture techniques which prohibit translation to the clinic. Our hESC lines have been manufactured in compliance with quality systems (cGMPs, cGTPs) required by international regulatory agencies for eventual human transplantation, and has a normal karyotype: 46,XY.

In addition to the standard tests of pluripotency and karyotype, we also provide complete genome sequence by Complete Genomics, Inc., the leading provider of genome technologies. Researchers can review the distribution of important transplantation alleles (ABO blood type) and disease propensity alleles (GWAS), and can also confirm the integrity of key tumor suppressor and oncogenes. This line is STR fingerprinted, allowing for absolute identification at any point during derivation or development. Finally, 10 locus HLA typing is available.

Please note: Embryonic Stem Cell Line ESI-017 has been extensively characterized and is available here as an economic research grade product. It is also available as a clinical grade product allowing efficient translation from clinical research to eventual clinical use. The product offered here is restricted for research use only and is not intended for human use.

Contents of package

  • 1 vial

Positive stem-cell markers:

NANOG, POU5F1, SOX2, SSEA-3 TRA-1-60

Experimental Data

  • ESI-049 was successfully induced to generate Cardiomyocytes1
  • ESI-049 was successfully induced to generate Endoderm and Pancreatic progenitor cells2, 3

Passage number:

ESI-049 is sold at passage 23

Substrate:

MFFs, HFFs, BD Matrigel™, or other preferred substrate for hES cells

Growing hES Cells:

Change media first four days with media containing Rock Inhibitor. Change media or passage every day thereafter with media NOT containing Rock Inhibitor. When the culture is 75-90% confluent, it must be passaged. Cells should be passaged at a ratio of 1:3 to 1:12 depending on specific cell line and intended use of the cells.

Availability:

In stock

References

1 Jeremy Micah Crook, Teija Tuulikki Peura, Lucy Kravets, Alexis Gina Bosman, Jeremy James Buzzard, Rachel Horne, Hannes Hentze, Norris Ray Dunn, Robert Zweigerdt, Florence Chua, Alan Upshall, and Alan Colman 2007 The Generation of Six Clinical-Grade Human Embryonic Stem Cell Lines. Cell Stem Cell 1, 490-494 2 Teo AK, Ali Y, Wong KY, Chipperfield H, Sadasivam A, Poobalan Y, Tan EK, Wang ST, Abraham S, Tsuneyoshi N, Stanton LW, Dunn NR 2012 Activin and BMP4 synergistically promote formation of definitive endoderm in human embryonic stem cells. Stem Cells 30(4):631-42 3 Rezania A, Bruin JE, Riedel MJ, Mojibian M, Asadi A, Xu J, Gauvin R, Narayan K, Karanu F, O'Neil JJ, Ao Z, Warnock GL, Kieffer TJ 2012 Maturation of human embryonic stem cell-derived pancreatic progenitors into functional islets capable of treating pre-existing diabetes in mice. Diabetes ;61(8):2016-29

Restrictions

These cells are provided for research purposes only. They are not for human use, and may not be used for commercial purposes. The user is responsible for proper handling upon receipt.
$940.00 $1,250.00  

Contains:

1 vial
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About HyStem Hydrogel

The HyStem Hydrogel is a xeno-free Hyaluronan-based hydrogel product designed for optimal culturing of cells. HyStem can be customized by adding extracellular matrix (ECM) proteins or cell attachment peptides into the hydrogel to provide attachment site and/or differentiation signals. HyStem Hydrogels can also be varied by changing the hydrogel rigidity to match that of the native cell environment.

HyStem Hydrogel is recommended for optimal differentiation for this product.

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Contents of package

  • 1 vial
Total: $940.00
$1,250.00
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