BioTime provides research grade versions of ﬁve independent embryonic stem cell lines that represent the best characterized and documented hESC lines available today. The ESI collection (ESI-017,035,049,051 and 053) was derived using procedures and documentation that are in compliance with cGTPs and cGMPs 1, and represents the most comprehensive collection of clinical grade lines available. Unlike other hESC lines, our ESI hESC lines were not developed using either live animal cells (such as mouse embryonic ﬁbroblast feeder cells) or unqualiﬁed animal-based products for culture techniques which prohibit translation to the clinic. Our hESC lines have been manufactured in compliance with quality systems (cGMPs, cGTPs) required by international regulatory agencies for eventual human transplantation, and has a normal karyotype: 46,XX.
In addition to the standard tests of pluripotency and karyotype, we also provide complete genome sequence by Complete Genomics, Inc., the leading provider of genome technologies. Researchers can review the distribution of important transplantation alleles (ABO blood type) and disease propensity alleles (GWAS), and can also conﬁrm the integrity of key tumor suppressor and oncogenes. This line is STR ﬁngerprinted, allowing for absolute identiﬁcation at any point during derivation or development. Finally, 10 locus HLA typing is available.
Please note: Embryonic Stem Cell Line ESI-017 has been extensively characterized and is available here as an economic research grade product. It is also available as a clinical grade product allowing efficient translation from clinical research to eventual clinical use. The product offered here is restricted for research use only and is not intended for human use.
- ESI-051 was successfully induced to generate Cardiomyocytes1
ESI-051 is sold at passage 32
MFFs, HFFs, BD Matrigel™, or other preferred substrate for hES cells
Collagenase, trypsin, microdissection, or other preferred dissociation method
Growing hES Cells:
Change media first four days with media containing Rock Inhibitor. Change media or passage every day thereafter with media NOT containing Rock Inhibitor. When the culture is 75-90% confluent, it must be passaged.
Cells should be passaged at a ratio of 1:3 to 1:12 depending on specific cell line and intended use of the cells.
1 Jeremy Micah Crook, Teija Tuulikki Peura, Lucy Kravets, Alexis Gina Bosman, Jeremy James Buzzard, Rachel Horne, Hannes Hentze, Norris Ray Dunn, Robert Zweigerdt, Florence Chua, Alan Upshall, and Alan Colman 2007 The Generation of Six Clinical-Grade Human Embryonic Stem Cell Lines. Cell Stem Cell 1, 490-494
These cells are provided for research purposes only. They are not for human use, and may not be used for commercial purposes. The user is responsible for proper handling upon receipt.