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PureStem™ Chondrogenic Package 4D20.8, NCr-fac Progenitor

CAT #: LM-CH-4D20.8-HY

The PureStem Chondrogenic Package 4D20.8 includes the human clonal progenitor line PureStem 4D20.8, NCr-fac Progenitor, specific growth media, a chondrogenic differentiation kit and Hystem Hydrogel. When following the recommended protocols, the differentiated cells express late markers specific to mature chondrocytes and their extracellular matrix, including type 2 collagens and proteoglycans.

HyStem HydroGel is recommended for obtaining optimal differentiation results (more information).

This package provides the basis for study of chondrogenesis along with cartilage/bone development and related diseases, such as degenerative disc disease and osteoarthrisis.

Contents of package

Differentially expressed positive mRNA markers (by microarray):

COL2A1, COL10A1, COMP, OGN, COL9A2, MGP, EPYC, LECT1, CILP

Experimental Data

  • This package was successfully applied to achieve in vitro differentiation of 4D20.8 to functional chondrocytes1
  • This package was successfully used to repair articular chondrocytes in the rat model - see below1

Growth Media:

Growth conditions provided in product data sheet. Growth media included in package. LifeMap recommends using Progenitor growth media EM-1001, which is optimized for growth of PureStem 4D20.8, NCr-fac Progenitor.

Differentiation Protocols:

Differentiation protocols are detailed in the product datasheet.

Availability:

3 weeks

References

1 Sternberg H, Murai JT, Erickson IE, Funk WD, Das S, Wang Q, Snyder E, Chapman KB, Vangsness CT Jr, West MD. 2012 A human embryonic stem cell-derived clonal progenitor cell line with chondrogenic potential and markers of craniofacial mesenchyme. Regen Med. 7(4):481-501 2 West MD, Sargent RG, Long J, Brown C, Chu JS, Kessler S, Derugin N, Sampathkumar J, Burrows C, Vaziri H, Williams R, Chapman KB, Larocca D, Loring JF, Murai J. 2008 The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308

Restrictions

This package and its components are provided for research purposes only. They are not for human use, and may not be used for commercial purposes. The user is responsible for proper handling upon receipt.
Starts at: $1,020.00 $1,074.00
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Product Highlights

  • High propagation capacity
  • Clonally pure population
  • Demonstrated in vitro chondrogenic differentiation
  • Demonstrated in vivo cartilage repair in the rat model

Applications:

  • Studying chondrogenesis and osteogenesis
  • Studying cartilage and bone-related diseases
LifeMap Discovery

The database of embryonic development stem cell research & regenerative medicine

Explore 4D20.8 in LifeMap Discovery™
About HyStem Hydrogel

The HyStem Hydrogel is a xeno-free Hyaluronan-based hydrogel product designed for optimal culturing of cells. HyStem can be customized by adding extracellular matrix (ECM) proteins or cell attachment peptides into the hydrogel to provide attachment site and/or differentiation signals. HyStem Hydrogels can also be varied by changing the hydrogel rigidity to match that of the native cell environment.

HyStem Hydrogel is recommended for optimal differentiation for this product.

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Contents of package

  • purestem 4d20 8 ncr fac progenitor
  • purestem chondrogenic differentiation kit em 2001
  • progenitor growth media em 1001
  • HyStem Hydrogel - Recommended for optimal differentiation
Total: $1,206.00
$1,269.00
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