SCIENTISTS ABOUT OUR PRODUCTS
  • "It helps to start with the purest cell lines possible to find cell targeting reagents, so we used PureStem™ Cells from BioTime, which are clonally pure having been expanded from a single cell. This greatly simplified our screening efforts."
    David Larocca, Ph.D.
    CEO
    Mandala Biosciences, LLC.
  • "The embryonic progenitors from BioTime were robust and easy to culture. We were able to show positive expression of desired protein markers indicating successful directed differentiation when using BioTime's culture reagents."
    Rachel Krupa
    Senior Research Associate
    Mandala BioSciences, LLC.
  • "The embryonic progenitors from BioTime were easy to grow and can be used for multiple purposes."
    Sheryasi Das
    Post Doctorial Scientist
    Sanford / Burnham Medical Research Institute
  • "Embryonic progenitor cells are much more plastic and offer the brightest future for cells used in regenerative medicine."
    C.T. Vangsness, M.D.
    Professor of Orthopaedic Surgery
    Keck Medical Center of USC

Home » Human Embryonic Progenitor

PureStem™ MEL2, NCr-fac Progenitor

CAT #: ES-268

The clonal human embryonic progenitor line MEL2 expresses chondrogenic (NKX3-2, SOX9, COL1A1) and osteogenic (SATB2, ALPL, MSX2) markers. In addition, it expresses markers of neural crest and lateral plate-derived head and limb mesenchymal cells (FRZB, HAND2, DLX5, DLX6, TWIST1, FOXD1, MSX2, TFAP2A, SOX9).

Progenitor line MEL2 was derived from the parental NIH registered Human ES cell line H9 (WA09) (West, M.D. et al, 2008) 1.

Differentially expressed positive mRNA markers (by microarray):

TWIST1, TSPAN18, TFAP2A, SOX9, SATB2, NPTX2, NKX3-2, MSX2, HAND2, GPR37, GPC3, FRZB, FOXD1, DLX6, DLX5, COL1A1, CHRNA1, CD24, ALPL, ALDH1A2, RASL12, PITX1

Selected cell surface markers:

PTH1R

Selected secreted factors:

APOD, BMP2, COL11A1, COL1A1, COL1A2, DCN, FRZB, OMD, POSTN, PRG4, SFRP2, TNC

Experimental Data

  • PureStem MEL2 was characterized by MicroArray analysis and compared to bone marrow-derived Mesenchymal Stem Cells - see heat map below.
  • PureStem MEL2 were induced to differentiate in vitro into functional chondrocytes.

Differentiation Potential:

Growth Media:

Optimal growth conditions and growth factors are detailed in the product datasheet. LifeMap recommends using progenitor growth media em 1004, which is optimized for growth of MEL2 mesenchymal progenitor cells.

Differentiation Protocols:

Chondrogenic differentiation protocol is detailed in respective differentiation kits' datasheet (purestem chondrogenic differentiation kit em 2001).

Propagation Potential:

Progenitor line MEL2 is sold at passage 17 (passage 1: original clonal isolate at confluence in 1.9 cm2 well), which corresponds to approximately ?? doublings since the first passage and approximately ?? doublings since its original clonal plating. The line displays a finite lifespan in vitro, and when properly maintained may reach [TBD] population doublings.

Quality Control:

The line is negative for HIV (1, 2), HBV, HCV, bacteria, mycoplasma, and fungal contamination. Certificates of analysis are available on request.

Availability:

In stock, no production lead times

References

1 West MD, Sargent RG, Long J, Brown C, Chu JS, Kessler S, Derugin N, Sampathkumar J, Burrows C, Vaziri H, Williams R, Chapman KB, Larocca D, Loring JF, Murai J. 2008 The ACTCellerate initiative: large-scale combinatorial cloning of novel human embryonic stem cell derivatives. Regen Med. 3(3):287-308

Restrictions

These cells are provided for research purposes only. They are not for human use, and may not be used for commercial purposes. The user is responsible for proper handling upon receipt.
$650.00  

Contains:

1 vial containing >500,000 cells
Phone us+1-(855)-888-7221

Product Highlights

  • High propagation capacity
  • Clonally pure population
  • Demonstrated in vitro chondrogenic differentiation

Applications:

  • Chondrogenesis and osteogenesis research
  • Cartilage and bone-related diseases research
LifeMap Discovery

The database of embryonic development stem cell research & regenerative medicine

Explore MEL2 in LifeMap Discovery™
About HyStem Hydrogel

The HyStem Hydrogel is a xeno-free Hyaluronan-based hydrogel product designed for optimal culturing of cells. HyStem can be customized by adding extracellular matrix (ECM) proteins or cell attachment peptides into the hydrogel to provide attachment site and/or differentiation signals. HyStem Hydrogels can also be varied by changing the hydrogel rigidity to match that of the native cell environment.

HyStem Hydrogel is recommended for optimal differentiation for this product.

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Contents of package

  • 1 vial containing >500,000 cells
Total: $650.00
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